Sami and Lola Sheen Unite at Eilish Premiere

Lola's second denial came one day after Sami kicked off the online drama with her explosive allegation about Lola allegedly being in a secret romance with her ex.

She made the claim on TikTok, but did not identify the former flame.

Sami claimed to have discovered the alleged relationship after going through her ex's phone and finding over 300 deleted text messages between him and Lola.

In the shocking video, Sami appeared stunned as she sat silently before the camera.

'Imagine finding out your sister and ex boyfriend have been seeing each other behind your back this whole time,' she wrote.

Sami claimed she found evidence of the alleged relationship after going through her ex's phone.

'Went through his phone and saw over 300 deleted texts between them,' she told one curious TikToker.

She also alleged her sister had given her ex a Bible.

In a lengthy statement shared just hours later, Lola hit back against the allegations and insisted there was no truth to them.

'I am never one to defend myself against my sister anymore because I genuinely don't care. But I couldn't let this one slide,' she posted to TikTok.

'I am single, I am not talking to anyone, and I'm definitely not dating her ex-boyfriend. That is absolutely the one thing I will share my side for because this is unbelievable.

'Not that this is anyone business, but the truth is my mom hired her ex boyfriend to take care of my moms dogs and basically clean our whole house, so he has been working for her almost everyday.'

'I was living with roommates, and had to move back home for the meantime until I move into another place. I was absolutely not speaking to her ex boyfriend, but then my mom allowed him to move into our office, across the hall from me.'

'I would absolutely NEVER do anything with my sister ex boyfriend. We have had a few conversations because it's hard to completely ignore someone who is in the same home as me now, which was NOT my choice. And we have completely different types in men.'

'This is not a situation I want to be in at all, but I truly have no control until God provides a different place for me to move into. I have absolutely not been seeing her ex boyfriend. That is a complete lie. You can believe whatever you want because I really don't know any of you personally, and only the people closest to me know that this is not true at all.'

'My sister has always posted things about me online to get people to hate on me, with absolutely no context at all.'

The sisters have certainly had a fractured relationship that has played out publicly - with the family's reality show, Denise Richards and her Wild Things - revealing they went a year without speaking to one another.

The series showed the siblings' rivalry and how their mother was attempting to repair their feud.

Last June, Sami accused Lola of being homophobic and transphobic in a series of emotional TikTok videos, alleging that Lola had reposted conservative Christian content she felt mocked LGBTQ and transgender people.

Sami said she chose to speak out publicly because the posts were visible to anyone and because she herself is part of the LGBTQ community.

'It's honestly disgusting, close-minded behavior,' she said at the time, urging her sister to reconsider her views.

Sami went on to urge Lola to 'put the bible down - it's gonna be OK, you can live without it for five minutes - and pick up a pride flag and support the f***ing community.'

'I don't know if you've realized, it is pride month, and you reposting those things during pride month is just otherworldly,' she continued as her fury escalated. 'I don't know who you are anymore. This is not you. We were not raised like this.

'And I am embarrassed. I am f***ing embarrassed that my own sister is a homophobe,' Sami added before signing off. 'Do better! Seriously!'